Pitter Huesgen
Professor for Biochemistry and Functional Proteomics (W3)
Research topic in GRK 2606: Improved C-terminomics for protease substrate discovery
Chemistry, 1998-2001, Marburg, Germany
MSc, Biochemistry, 2001-2002, Stockholm, Sweden2019 - 2023, Professor for Protein Dynamics and Proteolysis (W2), Cologne Excellence Cluster on Cellular Stress Responses in Aging-associated Diseases (CECAD), Medical Faculty and University Hospital, and Institute of Biochemistry, Department of Chemistry, University of Cologne, Germany
2014 - 2023, Team leader/PI protein mass spectrometry, ZEA-3 Analytics, Forschungszentrum Jülich (FZJ), Germany
2013 - 2014, Research Associate, Centre for Blood Research and Faculty of Dentistry, University of British Columbia, Vancouver, BC, Canada (Proteomics, Prof. Dr. C.M. Overall)
2008 - 2013, Postdoctoral Fellow, Centre for Blood Research and Faculty of Dentistry, University of British Columbia, Vancouver, BC, Canada (Proteomics, Prof. Dr. C.M. Overall)
2007 - 2008, Postdoc, Department of Biology, University of Konstanz, Germany (Plant biochemistry, Prof. Dr. I. Adamska).
| 2024 | Co-Organizer of the 6th Conference on Plant Proteases, Hohenheim |
| 2019 - 2023 | Member of the JuDOCS Council (PhD program board), Forschungszentrum Jülich |
| 2014 | Starting grant of the European Research Council |
| 2013 | Offer for an Assistant Professorship in Plant Biochemistry, Umeå Plant Science Centre, Umeå University, Sweden (declined) |
| 2010 - 2011 | Postdoctoral Fellowship, Michael Smith Foundation for Health Research, Canada |
| 2009 - 2010 | Postdoctoral Fellowship, German Academic Exchange Service (DAAD), Germany |
| 2008 | Nycomed Award for PhD thesis, Nycomed AG, Konstanz, Germany |
10 important publications
- Barghahn S#, Saridis G#, Mantz M#, Meyer U, Mellüh JC, Misas Villamil JC, Huesgen PF*, Doehlemann G* (2023) Combination of transcriptomic, proteomic and degradomic profiling reveals common and distinct patterns of pathogen-induced cell death in maize. Plant J. 2023 Jun 20. doi: 10.1111/tpj.16356.
- Demir F*, Kizhakkedathu JN, Rinschen MM, Huesgen PF* (2021) MANTI: Automated Annotation of Protein N-Termini for Rapid Interpretation of N-Terminome Data Sets. Anal Chem 93:5596-5605.
- Soh WT#, Demir F#, Dall E, Perrar A, Dahms SO, Kuppusamy M, Brandstetter H, Huesgen PF* (2020) ExteNDing proteome coverage with legumain as highly specific digestion protease. Anal Chem 92:2961-2971.
- Hofsetz E, Demir F, Szczepanowska K, Kukat A, Kizhakkedathu J, Trifunovic A*, Huesgen PF* (2020) The mouse heart mitochondria N terminome provides insights into ClpXP-mediated proteolysis. Mol Cell Proteomics 19:1330-1345.
- Weng SSH#, Demir F#, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF*, Lange PF* (2019) Sensitive determination of proteolytic proteoforms in limited microscale proteome samples. Mol Cell Proteomics18:2335-2347.
- Misas-Villamil J, Mueller AN, Demir F, Meyer U, Breuer M, Dauben H, Win J, Huesgen PF, Doehlemann G (2019) The fungal effector Pit2 acts as molecular mimicry to suppress plant immunity via an inter-kingdom conserved motif. Nat Commun 10:1576.
- Rinschen MM, Hoppe AK, Grahammer F, Kann M, Völker LA, Schurek EM, Binz J, Höhne M, Malisic M, Demir F, Malisic M, Huber TB, Kurschat C, Kizhakkedathu JN, Schermer B, Huesgen PF*, Benzing T* (2017) N-Degradomic analysis reveals a proteolytic network processing the podocyte cytoskeleton. J Am Soc Nephrol, 28(10):2867-2878.
- Eckhard U#, Huesgen PF#, Schilling O#, Bellac CL, Butler GS, Cox JH, Dufour A, Goebeler V, Kappelhoff R, auf dem Keller U, Klein T, Lange PF, Marino G, Morrison CJ, Prudova A, Rodriguez D, Starr AE, Wang Y Overall CM (2016) Active site specificity of the Matrix Metalloproteinase family: Proteomic identification of 4,300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses. Matrix Biol, 49:37-90.
- Huesgen PF, Lange PF, Rogers LR, Solis N, Eckhard U, Kleifeld O, Gomis-Rüth XF, Overall CM (2015) LysargiNase mirrors trypsin for protein C-termini and methylation site identification. Nature Methods, 12:55-8.
- Schilling O, Barré O, Huesgen PF, Overall CM (2010) Proteome-wide Characterization of Protein Carboxy-Termini: C-terminomics. Nature Methods 7:508-11.