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Svenja Wöhrle

Name Svenja Wöhrle
Education/Training Master of Sience, Molecular Cell Biology and Immunology
Start of PhD Thesis and Group 06/20, Prof. Olaf Groß
Subject of Thesis Nlrp3 inflammasome activation by small molecules
Research Interests Immunology, Nlrp3 inflammasome, DPP9 Inhibitors
(Studying inflammasome biology on the molecular, cellular and organismal level, to understand activation under different conditions)
Methods and Techniques - Basic cell culture techniques
- Isolation and Cultivation primary cells
- Inflammasome assay
- Flow cytometry analysis
- Biochemical analysis (Western Blot, ELISA)
- Confocal microscopy and live cell imaging
- Molecular biology methods ( DNA, mRNA isolation, qPCR, PCR, ATAC   Sequencing, plasmid cloning, viral transfection)
 - Metabolic analysis (mitochondrial respiration and glycolysis)
Publications

Neuwirt E, Magnani G, Ćiković T, Wöhrle S, Fischer L, Kostina A, Flemming S, Fischenich NJ, Saller BS, Gorka O, Renner S, Agarinis C, Parker CN, Boettcher A, Farady CJ, Kesselring R, Berlin C, Backofen R, Rodriguez-Franco M, Kreutz C, Prinz M, Tholen M, Reinheckel T, Ott T, Groß CJ, Jost PJ, Groß O. Tyrosine kinase inhibitors can activate the NLRP3 inflammasome in myeloid cells through lysosomal damage and cell lysis. Sci Signal. 2023 Jan 17;16(768):eabh1083. doi: 10.1126/scisignal.abh1083. Epub 2023 Jan 17. DOI: 10.1126/scisignal.abh108

Carvalho LAR, Ross B, Fehr L, Bolgi O, Wöhrle S, Lum KM, Podlesainski D, Vieira AC, Kiefersauer R, Félix R, Rodriguez T, Lucas SD, Groß O, Geiss-Freidlander R, Cravatt BF, Huber R, Kaiser M, Moreira R. Chemoproteomics-Enabled Identification of 4-Oxo-β-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9. Angew. Chem.Int. Ed.2022,61,e202210498. https://doi.org/10.1002/anie.202210498

Contact svenja.woehrle[at]uniklinik-freiburg.de