Svenja Wöhrle
| Name | Svenja Wöhrle |
| Education/Training | Master of Sience, Molecular Cell Biology and Immunology |
| Start of PhD Thesis and Group | 06/20, Prof. Olaf Groß |
| Subject of Thesis | Nlrp3 inflammasome activation by small molecules |
| Research Interests | Immunology, Nlrp3 inflammasome, DPP9 Inhibitors (Studying inflammasome biology on the molecular, cellular and organismal level, to understand activation under different conditions) |
| Methods and Techniques | - Basic cell culture techniques - Isolation and Cultivation primary cells - Inflammasome assay - Flow cytometry analysis - Biochemical analysis (Western Blot, ELISA) - Confocal microscopy and live cell imaging - Molecular biology methods ( DNA, mRNA isolation, qPCR, PCR, ATAC Sequencing, plasmid cloning, viral transfection) - Metabolic analysis (mitochondrial respiration and glycolysis) |
| Publications |
Neuwirt E, Magnani G, Ćiković T, Wöhrle S, Fischer L, Kostina A, Flemming S, Fischenich NJ, Saller BS, Gorka O, Renner S, Agarinis C, Parker CN, Boettcher A, Farady CJ, Kesselring R, Berlin C, Backofen R, Rodriguez-Franco M, Kreutz C, Prinz M, Tholen M, Reinheckel T, Ott T, Groß CJ, Jost PJ, Groß O. Tyrosine kinase inhibitors can activate the NLRP3 inflammasome in myeloid cells through lysosomal damage and cell lysis. Sci Signal. 2023 Jan 17;16(768):eabh1083. doi: 10.1126/scisignal.abh1083. Epub 2023 Jan 17. DOI: 10.1126/scisignal.abh108 Carvalho LAR, Ross B, Fehr L, Bolgi O, Wöhrle S, Lum KM, Podlesainski D, Vieira AC, Kiefersauer R, Félix R, Rodriguez T, Lucas SD, Groß O, Geiss-Freidlander R, Cravatt BF, Huber R, Kaiser M, Moreira R. Chemoproteomics-Enabled Identification of 4-Oxo-β-Lactams as Inhibitors of Dipeptidyl Peptidases 8 and 9. Angew. Chem.Int. Ed.2022,61,e202210498. https://doi.org/10.1002/anie.202210498 Saller BS, Wöhrle S, Fischer L, Dufossez C, Ingerl IL, Kessler S, Mateo-Tortola M, Gorka O, Lange F, Cheng Y, Neuwirt E, Marada A, Koentges C, Urban C, Aktories P, Reuther P, Giese S, Kirschnek S, Mayer C, Pilic J, Falquez-Medina H, Oelgeklaus A, Deepagan VG, Shojaee F, Zimmermann JA, Weber D, Tai YH, Crois A, Ciminski K, Peyronnet R, Brandenburg KS, Wu G, Baumeister R, Heimbucher T, Rizzi M, Riedel D, Helmstädter M, Buescher J, Neumann K, Misgeld T, Kerschensteiner M, Walentek P, Kreutz C, Maurer U, Rambold AS, Vince JE, Edlich F, Malli R, Häcker G, Kierdorf K, Meisinger C, Köttgen A, Jakobs S, Weber ANR, Schwemmle M, Groß CJ, Groß O. Acute suppression of mitochondrial ATP production prevents apoptosis and provides an essential signal for NLRP3 inflammasome activation. Immunity. 2024 Nov 15:S1074-7613(24)00492-8. doi: 10.1016/j.immuni.2024.10.012. Epub ahead of print. PMID: 39571574. Vogele D, Wöhrle S, Saller BS, Fröhlich K, Barta BA, Cosenza-Contreras M, Groß O, Schilling O. Size exclusion chromatography based proteomic and degradomic profiling of inflammasome-activated, murine bone marrow-derived dendritic cells highlights complex retention and release of cleavage products. Mol Omics. 2024 Oct 28;20(9):595-610. doi: 10.1039/d4mo00163j. PMID: 39378052; PMCID: PMC11460583. |
| Contact | svenja.woehrle[at]uniklinik-freiburg.de |